INTRODUCTION TO UPSTREAM PROCESSING
Introduction to Upstream Processing will include both hands-on practical and theory components covering cell vial thaw, aseptic techniques, cell counting, metabolic analysis, scale up, and bioreactor operation. Equipment such as wave bag reactors, stirred-tank reactors, as well as single use bioreactors will all be utilized to reflect the current state of biopharmaceutical manufacturing technology.
This course is designed for operators, technical staff, engineers, and managers wishing to increase their knowledge of the upstream operations in a modern bioprocessing plant.
- Overview of the biopharmaceutical production process
- Mammalian cell culture aseptic techniques
- Analysis of cell growth and nutrients in culture
- Cell culture scale up equipment including shake flasks and disposable bioreactors
- Aseptically set-up and operate a 200L bioreactor
- Strategies for media preparation in single-use bioreactors
Upon completion of this module the learner will be able to:
- Describe the practice of culturing mammalian cells in an aseptic environment from small to large scale
- List the equipment required for scale up of mammalian cultures
- Describe the cell expansion and inoculation methods in single-use systems
- Use GDocP to follow and complete SOPs, batch records and log books
- Count cells using both an automated and manual method
- Interpret the nutrient and metabolite levels of cell culture medium
- Aseptically prepare and run a 200L single-use bioreactor
A competency-based assessment will be undertaken at the end of the course. This will certify proﬁciency in the theory and practical skills.
COURSE DELIVERY MODEL
1 day of theoretical instructor-led virtual classroom
2 days practical at CASTL training facility in Charlottetown
1 day Theory
THEORY & PRACTICAL
1 day Theory + 2 days Practical