INTRODUCTION TO UPSTREAM PROCESSING
Short Courses / Introduction to Upstream Processing
COURSE DESCRIPTION
Introduction to Upstream Processing will include both hands-on practical and theory components covering cell vial thaw, aseptic techniques, cell counting, metabolic analysis, scale up, and bioreactor operation. Equipment such as wave bag reactors, stirred-tank reactors, as well as single use bioreactors will all be utilized to reflect the current state of biopharmaceutical manufacturing technology.
Current Offerings:
Theory: May 11-12, 2023 – Practical: May 15-16, 2023
Theory: September 14-15, 2023 – Practical: September 25-26, 2023
AUDIENCE
This course is designed for operators, technical staff, engineers, and managers wishing to increase their knowledge of the upstream operations in a modern bioprocessing plant.
TOPICS
- Overview of the biopharmaceutical production process
- Mammalian cell culture aseptic techniques
- Analysis of cell growth and nutrients in culture
- Cell culture scale up equipment including shake flasks and disposable bioreactors
- Aseptically set-up and operate a 200L bioreactor
- Strategies for media preparation in single-use bioreactors
LEARNING OBJECTIVES
Upon completion of this module the learner will be able to:
- Describe the practice of culturing mammalian cells in an aseptic environment from small to large scale
- List the equipment required for scale up of mammalian cultures
- Describe the cell expansion and inoculation methods in single-use systems
- Use GDocP to follow and complete SOPs, batch records and log books
- Count cells using both an automated and manual method
- Interpret the nutrient and metabolite levels of cell culture medium
- Aseptically prepare and run a 200L single-use bioreactor
ASSESSMENT
A competency-based assessment will be undertaken at the end of the course. This will certify proficiency in the theory and practical skills.
COURSE DELIVERY MODEL
2 mornings of theoretical instructor-led virtual classroom
2 days practical at CASTL Biomanufacturing Training Facility in Charlottetown
THEORY ONLY
1 day Theory
$1,395.00 +HST
THEORY & PRACTICAL
1 day Theory + 2 days Practical
$4,195.00 +HST